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An aqueous extract of Vitex agnus castus alters the labeling of blood constituents with technetium-99m BABT
Costa,Maria Regina de Macedo; Ribeiro,Camila Godinho; Santos-Filho,Sebastião David; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida.
The development of experimental assays to study properties of herbal medicine is worthwhile. Vitex agnus castus (VAC) is utilized in popular medicine and some actions have been attributed to its extract. Blood cells (BC) and plasma proteins are labeled with technetium-99m (Tc-99m) and have been used in nuclear medicine, as in basic research. This procedure uses a reducing agent and stannous ion is utilized. There are reports that drugs can alter this labeling process. The aim of this work was to evaluate the influence of an aqueous extract of VAC on the labeling of blood constituents with Tc-99m. Blood was incubated with VAC, stannous chloride and Tc-99m, as sodium pertechnetate, and centrifuged. Samples of BC and plasma were separated, aliquots of BC and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxidant properties; Plasma proteins; Red blood cells; Technetium-99m; Vitex agnus-castus.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600023
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Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells BABT
Rebello,Bernardo Machado; Moreno,Silvana Ramos Farias; Ribeiro,Camila Godinho; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Caldas,Luis Querino de Araújo; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Labeling; Blood; Passiflora edulis f. flavicarpa; Radiobiocomplexes; Technetium-99m.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600019
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Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells BABT
Neves,Rosane de Figueiredo; Moreno,Silvana Ramos Farias; Rebello,Bernardo Machado; Caldas,Luiz Querino de Araújo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arctium lappa; Technetium-99m; Blood constituents; Morphometry.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600021
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Evaluation of the effect of an extract of sabugueiro (Sambucus australis) on the labeling of blood constituents with technetium-99m BABT
Ribeiro,Camila Godinho; Rebello,Bernardo Machado; Neves,Rosane de Figueiredo; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida.
Sambucus australis (sabugueiro) has been used to treat inflammatory and rheumatologic disorders. Blood constituents labeled with technetium-99m (99mTc) have been used in nuclear medicine to obtain diagnostic images. The aim of this work was to evaluate the effect of a sabugueiro extract on the labeling of blood cells with 99mTc. Blood samples from Wistar rats were incubated with sabugueiro extract and the radiolabeling assay of blood constituents was carried out. After centrifugation, samples of plasma and blood cells were separated. Aliquots of plasma and blood cells were precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions. The radioactivity in each fraction was counted and the percentage of activity (%ATI)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sambucus australis; Blood constituents; Technetium-99m.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600020
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Nuclear medicine imaging technique in the erectile dysfunction evaluation: a mini-review BABT
Ribeiro,Camila Godinho; Moura,Regina; Neves,Rosane de Figueiredo; Spinosa,Jean Pierre; Bernardo-Filho,Mario.
Functional imaging with positron emission tomography and single photon emission computed tomography is capable of visualizing subtle changes in physiological function in vivo. Erectile dysfunction(ED) diminishes quality of life for affected men and their partners. Identification of neural substrates may provide information regarding the pathophysiology of types of sexual dysfunction originating in the brain. The aim of this work is to verify the approaches of the nuclear medicine techniques in the evaluation of the erectile function/disfunction. A search using the words ED and nuclear medicine, ED and scintigraphy, ED and spect and ED and pet was done in the PubMed. The number of citations in each subject was determined. Neuroimaging techniques offer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nuclear medicine; Erectile dysfunction; Positron emission tomography; Single photon emission computed tomography.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600010
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